Amount of substance, also called material quantity, measures the number of particles in a sample. The Units is mole( symbol mol). The unit is based on the mass of 0.012 kilogram (kg), of carbon 12 (C-12). One mole is called the Avogadro constant, equal to 6.02214129(30)×1023.
Amount of substance (n)=Mass (m)/Molar mass (M)
For example, when Mass is 20, Molar mass is 2, then Amount of substance is 10.